《植物生理学报》 2009, 45(3): 270-272

低温层积与外源GA₃ 对肉苁蓉种子萌发及其内源GA 和ABA 含量的影响

陈庆亮^1,2, 王华磊², 王志芬¹, 单成钢¹, 翟志席², 郭玉海^2,*

山东省农业科学院原子能农业应用研究所, 济南 250100; 2中国农业大学农学与生物技术学院中药材研究中心, 北京 100193

通信作者：郭玉海；E-mail: yhguo@cau.edu.cn；Tel: 010-62732556

摘　要：

研究低温层积对肉苁蓉种子胚率、内源赤霉素(GA)和脱落酸(ABA)含量以及外源赤霉素(GA3)对低温层积不同时间种子萌发率影响的结果表明: 低温层积可以提高种子的胚率及其GA的含量, 降低其ABA含量; 层积120~150 d的肉苁蓉种子经外源GA3 处理后, 其萌发率可以达到70％以上; 层积120 d是外源GA3 促进种子萌发的临界点。

关键词：肉苁蓉; 低温层积; 种子萌发; 赤霉素(GA); 脱落酸(ABA)

收稿：2008-11-03   修定：2008-12-05

资助：北京市科技计划(D07060200880701)、河北省科技攻关项目(03276408D-4)和重大基础研究前期研究专项(2004CCA01200)。

Effects of Cold Stratification and Exogenous Gibberellic Acid (GA₃) on Seed Germination and Contents of Endogenous Gibberellins (GAs) and Abscisic Acid (ABA) in Cistanche deserticola Y. C. Ma

CHEN Qing-Liang^1,2, WANG Hua-Lei², WANG Zhi-Fen¹, SHAN Cheng-Gang¹, ZHAI Zhi-Xi², GUO Yu-Hai^2,*

¹Institute for Application of Atomic Energy in Agriculture, Shandong Academy of Agricultural Sciences, Jinan 250100, China;²Chinese Medicinal Herbs Research Center, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China

Corresponding author: GUO Yu-Hai; E-mail: yhguo@cau.edu.cn; Tel: 010-62732556

Abstract:

The effects of cold stratification on embryo ratio and contents of endogenous GA and ABA in the seeds of Cistanche deserticola were investigated, and the effects of exogenous GA₃ on the germination rate of C. desertcola seeds at different stages of cold stratification were also studied. The results indicated that cold stratification increased the embryo ratio and the contents of GAs, but reduced the content of ABA in the seeds of C. deserticola. The germination rate of C. desertcola seeds stratified for 120 to 150 d were more than 70% with the treatment of exogenous GA₃. The critical point of C.desertcola seeds germination rate was in 120 d after cold stratification with the treatment of exogenous GA₃.

Key words: Cistanche deserticola; cold stratification; seed germination; GA; ABA